Scott, D.C., Chittori, S., Purser, N., King, M.T., Maiwald, S.A., Churion, K., Nourse, A., Lee, C., Paulo, J.A., Miller, D.J., Elledge, S.J., Harper, J.W., Kleiger, G., and Schulman, B.A. (2024) Structural basis for C-degron selectivity across KLHDCX-family E3 ubiquitin ligases. Nature Communications,Nov 15;15(1):9899.

Li, J., Purser, N., Liwocha, J., Scott, D.C., Byers, H.A., Steigenberger, B., Hill, S., Tripathi-Giesgen, I., Hinkle, T., Hansen, F.M.Prabu JR, Radhakrishnan SK, Kirkpatrick DS, Reichermeier KM, Schulman BA, and *Kleiger G. (2024). Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting. Molecular Cell. Apr 4;84(7):1304-1320. *corresponding author

Liwocha, J., Li, J., Purser, N., Rattanasopa, C., Maiwald, S., Krist, D.T., Scott, D.C., Steigenberger, B., Prabu, J.R., Schulman, B.A., and *Kleiger G. (2024). Mechanism of millisecond Lys48-linked poly-ubiquitin chain formation by cullin-RING ligases. Nature Structural and Molecular Biology 31, 378-389. 10.1038/s41594-023-01206-1. *corresponding author

Chrustowicz J, Sherpa D, Li J, Langlois CR, Papadopoulou EC, Vu DT, Hehl LA, Karayel Ö, Beier V, von Gronau S, Müller J, Prabu JR, Mann M, Kleiger G, Alpi AF, Schulman BA. (2023) Multisite phosphorylation dictates selectivce E2-E3 pairing as revealed by Ubc8/UBE2H-GID/CTLH assemblies. Molecular Cell 84, 293-308.

Purser N, Tripathi-Giesgen I, Li J, Scott DC, Horn-Ghetko D, Baek K, Schulman BA, Alpi AF, *Kleiger G. (2023) Catalysis of non-canonical protein ubiquitylation by the ARIH1 ubiquitin ligase. The Biochemical Journal 29;480(22):1817-1831. *corresponding author

Scott DC, King MT, Baek K, Gee CT, Kalathur R, Li J, Purser N, Nourse A, Chai SC, Vaithiyalingam S, Chen T, Lee RE, Elledge SJ, Kleiger G, and Schulman BA (2023) E3 ligase autoinhibition by C-degron mimicry maintains C-degron substrate fidelity. Molecular Cell 83, 1-17.

Ibarra R, Borror HR, Hart B, Gardner RG, and *Kleiger G (2021) The San1 Ubiquitin Ligase Avidly Recognizes Misfolded Proteins through Multiple Substrate Binding Sites. Biomolecules 11, 1619. https://doi.org/10.3390/biom11111619. *corresponding author

Horn-Ghetko D, Krist DT, Prabu JR, Baek K, Mulder MPC, Klügel M, Scott DC, Ovaa H, Kleiger G and Schulman BA (2021) Ubiquitin ligation to F-box protein targets by SCF-RBR E3-E3 super-assembly. Nature. 590(7847), 671-6.

Liwocha J, Krist DT, van der Heden van Noort GJ, Hansen FM, Truong VH, Karayel O, Purser N, Houston D, Burton N, Bostock MJ, Sattler M, Mann M, Harrison JS, *Kleiger G, *Ovaa H, *Schulman BA (2020) Linkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler. Nature Chemical Biology. 17(3), 272-9. *co-corresponding author

Scott DC and Kleiger G (2020) Regulation of Cullin-RING E3 ligase dynamics by Inositol hexakisphosphate. PNAS. 117(12):6292-6294.

Baek K, Krist DT, Prabu JR, Hill S, Klügel M, Neumaier LM, von Gronau S, Kleiger G, Schulman BA (2020) NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligase assembly. Nature. 578(7795), 461-466.

Hill S, Reichermeier K, Scott DC, Samentar L, Coulombe-Huntington J, Izzi L, Tang X, Ibarra R, Bertomeu T, Moradian A, Sweredoski MJ, Caberoy N, Schulman BA, Sicheri F, Tyers M, *Kleiger G (2019) Robust cullin-RING ligase function is established by a multiplicity of polyubiquitylation pathways. eLife, e51163. *corresponding author

Jones RD, Enam C, Ibarra R, Borror HR, Mostoller KE, Fredrickson EK, Lin J, Chuang E, March Z, Shorter J, Ravid T, Kleiger G, Gardner RG (2019) The extent of Ssa1/Ssa2 Hsp70 chaperone involvement in nuclear protein quality control degradation varies with substrate. Mol Biol Cell, 31(3):221-233.

Hill S, Hill C, Kleiger G (2018) Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for Their Ubiquitin Ligase Partners. Methods Mol Biol. 1844:39-58.

Hill S and Kleiger G (2017) Self-regulating Ubiquitin Ligases. EMBO J, published online January 13, 2017

Kleiger G and Deshaies R (2016) Tag Team Ubiquitin Ligases. Cell, 166 (5), 1080-1081.

Ibarra R, Sandoval D, Fredrickson EK, Gardner RG, *Kleiger G (2016) The San1 Ubiquitin Ligase Functions Preferentially with Ubiquitin-conjugating Enzyme Ubc1 during Protein Quality Control. Journal of Biological Chemistry, 291 (36), 18778-90. *corresponding author

Hill S, Harrison JS, Lewis SM, Kuhlman B, *Kleiger G (2016) Mechanism of Lysine 48 Selectivity during Poly-ubiquitin Chain Formation by the Ube2R1/2 Ubiquitin-Conjugating Enzyme. Molecular and Cellular Biology, 36 (11), 1720-32. PMC4959314. *corresponding author

Sandoval D, Hill S, Ziemba A, Lewis S, Kuhlman B, *Kleiger G (2014) Ubiquitin-conjugating Enzyme Cdc34 and Ubiquitin Ligase Skp1-Cullin-F-box Ligase (SCF) Interact through Multiple Conformations. Journal of Biological Chemistry, 290 (2), 1106-18. PMC4294478. *corresponding author

Kleiger G, *Mayor T. (2014) Perilous journey: a tour of the ubiquitin-proteasome system. Trends in Cell Biology, 24(6), 352-359. PMC4037451. *co-corresponding authors

Huang H, Ceccarelli DF, Orlicky S, St-Cyr DJ, Ziemba A, Garg P, Plamondon S, Auer M, Sidhu S, Marinier A, Kleiger G, Tyers M, Sicheri F (2013) E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Nature Chemical Biology, 10 (2), 156-63. PMC3905752

Ziemba A, Hill S, Sandoval D, Webb K, Bennett EJ, *Kleiger G (2013) Multimodal mechanism of action for the Cdc34 acidic loop: a case study for why ubiquitin-conjugating enzymes have loops and tails. Journal of Biological Chemistry, 288 (48), 34882-96. PMC3843100. *corresponding author

Besten Wd, Verma R, Kleiger G, Oania RS, Deshaies RJ (2012) NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nature Structural and Molecular Biology, 19 (5), 511-6. PMC3348432.

Saha A, Lewis S, Kleiger G, Kuhlman B, and Deshaies RJ (2011) Essential role for ubiquitin-ubiquitin-conjugating enzyme interaction in ubiquitin discharge from Cdc34 to substrate. Molecular Cell, 42 (1), 75-83. PMC3091889.

Tyrrell A, Flick K, Kleiger G, Zhang H, Deshaies RJ, Kaiser P (2010) Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes poly-ubiquitylated proteins. PNAS, 107 (46), 19796-801. PMC29993400.

Pierce NW, Kleiger G, Shan S, and Deshaies RJ (2009) Detection of sequential poly-ubiquitylation on a millisecond timescale. Nature, 462 (7273), 615-9. PMC2791906.

Kleiger G, Saha A, Lewis S, Kuhlman B, and Deshaies RJ (2009) Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of Cullin-RING ubiquitin ligase substrates. Cell, 139 (5), 957-968. PMC2804849.

Kleiger G, Hao B, Mohl DA, and Deshaies RJ (2009) A kinetic analysis of the ubiquitin conjugating enzyme Cdc34 reveals that the acidic C-terminal tail functions in both binding to and catalysis with SCF(Cdc4). JBC, 284 (52), 36012-23. PMC2794717.

Petroski MD, Kleiger G, and Deshaies, RJ (2006) Evaluation of a diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complex. Molecular Cell, 24 (4), 523-34. PMID17114057.

Kleiger G, Panina EM, Mallick P, Eisenberg D (2004) PFIT and PFRIT: bioinformatic algorithms for detecting glycosidase function from structure and sequence. Protein Science, 13 (1), 221-9. PMC2286537.

McCarty AS, Kleiger G, Eisenberg D, Smale ST (2003) Selective dimerization of a C2H2 zinc finger subfamily. Molecular Cell, 11(2), 459-70. PMID12620233

Kleiger G, Eisenberg D (2002) GXXXG and GXXXA motifs stabilize FAD and NAD(P)-binding Rossmann folds through C(alpha)-H…O hydrogen bonds and Van der waals interactions. Journal of Molecular Biology, 323 (1), 69-76. PMID12368099.

Kleiger G, Grothe, R, Mallick, P, and Eisenberg D (2002) GXXXG and AXXXA: common alpha-helical interaction motifs in proteins, particularly extremophiles. Biochemistry, 41 (19), 5990-7. PMID11993993.

Kleiger G, Perry J, and Eisenberg D (2001) 3D structure and significance of the GXXXG helical packing motif in tetramers of the E1β subunit of pyruvate dehydrogenase from the archeon Pyrobaculum aerophilum. Biochemistry, 40 (48),14484-14492. PMID11724561.

Cobb BS, Morales-Alcelay S, Kleiger G, Brown KE, Fisher AG, Smale ST (2000) Targeting of Ikaros to pericentromeric heterochromatin by direct DNA binding. Genes and Development, 14 (17), 2146-60. PMC316893

Kleiger G, Beamer, LJ, Grothe, R, Mallick, P, and Eisenberg D (2000) The 1.7 Angstrom crystal structure of BPI: a study of how two dissimilar amino acid sequences adopt the same fold. Journal of Molecular Biology, 299 (4), 1019-34. PMID10843855.